2C7J
Phycoerythrocyanin from Mastigocladus laminosus, 295 K, 3.0 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 295 |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS, MULTIWIRE |
| Spacegroup name | P 63 |
| Unit cell lengths | 156.745, 156.745, 40.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 51.300 - 3.000 |
| R-factor | 0.1894 |
| Rwork | 0.189 |
| R-free | 0.25950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SEE REMARK |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.208 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | BEAST |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.300 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.130 | 0.330 |
| Number of reflections | 9690 | |
| <I/σ(I)> | 5 | 2.2 |
| Completeness [%] | 85.7 | 61.2 |
| Redundancy | 3 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 277 | 5% PEG 4000, 5 MM POTASSIUM PHOSPHATE, PH 8.5, 4 DEG C, 4 MICRO-LITER OF 20 MG/ML PROTEIN, HANGING DROP |
