2C6Z
crystal structure of dimethylarginine dimethylaminohydrolase I in complex with citrulline
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-25 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.652, 80.134, 44.932 |
| Unit cell angles | 90.00, 108.07, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.200 |
| R-factor | 0.1215 |
| R-free | 0.16880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h70 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.030 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.200 |
| High resolution limit [Å] | 1.200 | 1.160 |
| Rmerge | 0.080 | 0.370 |
| Number of reflections | 91675 | |
| <I/σ(I)> | 10.8 | 2 |
| Completeness [%] | 98.0 | 77.9 |
| Redundancy | 2.98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 100 MM CITRIC ACID/NAOH, 20-40% PEG 8000, 2 MM TCEP, PH 5.0 |
