2C6P
Membrane-bound glutamate carboxypeptidase II (GCPII) in complex with phosphate anion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 261 |
| Detector technology | CCD |
| Collection date | 2003-12-01 |
| Detector | MARRESEARCH |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 102.573, 130.365, 159.414 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.140 - 2.390 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.25100 |
| Structure solution method | OTHER |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.714 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.140 |
| High resolution limit [Å] | 2.400 |
| Number of reflections | 42414 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.25 | 20 MM HEPES PH 7.25, 200 MM NACL, 5% (W/V) PEG 400 AND 15% (W/V) PEG 1500 |
