2C5U
T4 RNA Ligase (Rnl1) Crystal Structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 105.050, 39.870, 108.540 |
| Unit cell angles | 90.00, 117.27, 90.00 |
Refinement procedure
| Resolution | 27.780 - 2.210 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.25800 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.060 | 0.180 |
| Number of reflections | 40793 | |
| <I/σ(I)> | 33 | 7.5 |
| Completeness [%] | 98.6 | 86.1 |
| Redundancy | 8.2 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% PEG 3350, 0.2M CACL2 |
