2C5R

The structure of phage phi29 replication organizer protein p16.7 in complex with double stranded DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM16
Synchrotron site	ESRF
Beamline	BM16
Temperature [K]	173
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	65.505, 72.111, 127.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.900
R-factor	0.254
Rwork	0.251
R-free	0.29200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bnk
RMSD bond length	0.028
RMSD bond angle	2.218
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.060
High resolution limit [Å]	2.900	2.900
Rmerge	0.060	0.360
Number of reflections	12368
<I/σ(I)>	8	2.1
Completeness [%]	96.2	90.7
Redundancy	4.9	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1