2C5Q
Crystal structure of yeast YER010Cp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 128.193, 255.073, 48.173 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.940 - 1.700 |
| R-factor | 0.156 |
| Rwork | 0.155 |
| R-free | 0.18300 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.524 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.120 | |
| Number of reflections | 956280 | |
| <I/σ(I)> | 12.2 | |
| Completeness [%] | 99.3 | |
| Redundancy | 5.49 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 18% PEG 8K, 0.1M SODIUM CACODYLATE PH6, 0.1M MAGNESIUM SULPHATE, pH 6.00 |
