2C5J
N-terminal domain of tlg1, domain-swapped dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-06-30 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.666, 46.104, 117.887 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.930 - 2.100 |
| R-factor | 0.259 |
| Rwork | 0.256 |
| R-free | 0.31100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c5i |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.360 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.050 | 0.330 |
| Number of reflections | 14293 | |
| <I/σ(I)> | 16.4 | 4.5 |
| Completeness [%] | 96.4 | 91.9 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 20MG/ML PROTEIN, 8% PEG8000, 80MM K PHOSPHATE PH5.6, FROZEN IN 22.5% GLYCEROL, pH 5.60 |
