2C5I
N-terminal domain of tlg1 complexed with N-terminus of vps51 in distorted conformation
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-14 |
| Detector | ADSC CCD |
| Wavelength(s) | 0.939,0.981 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 65.776, 65.776, 62.577 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 56.800 - 2.300 |
| R-factor | 0.229 |
| Rwork | 0.226 |
| R-free | 0.29600 |
| Structure solution method | MAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.745 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0016) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.120 | 1.000 |
| Number of reflections | 7108 | |
| <I/σ(I)> | 24 | 4.1 |
| Completeness [%] | 99.6 | 99.1 |
| Redundancy | 21 | 22 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 8 MG/ML PROTEIN, 66% SATURATED AMMONIUM SULPHATE, 100 MM NA CITRATE PH 5.5 WITH A 3-FOLD MOLAR EXCESS OF THE PEPTIDE, 20% GLYCEROL FOR FREEZING |
