2C4C
Crystal structure of the NADPH-treated monooxygenase domain of MICAL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.709, 89.915, 83.573 |
| Unit cell angles | 90.00, 113.82, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.900 |
| R-factor | 0.246 |
| Rwork | 0.243 |
| R-free | 0.29400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.015 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.990 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.170 | 0.940 |
| Number of reflections | 25263 | |
| <I/σ(I)> | 10.5 | 2.5 |
| Completeness [%] | 98.4 | 94.5 |
| Redundancy | 6.8 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | pH 5.00 |
