2C4A
Structure of Neuraminidase Subtype N9 Complexed with 30 MM Sialic Acid (NANA, NEU5AC), Crystal Soaked for 3 Hours at 291 K.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200H |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-06-10 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 181.336, 181.336, 181.336 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.100 - 2.150 |
| R-factor | 0.16 |
| Rwork | 0.158 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7nn9 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.602 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.660 | 2.210 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.120 | 0.270 |
| Number of reflections | 27874 | |
| <I/σ(I)> | 5.5 | 2.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.9 | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | PROTEIN WAS CRYSTALLISED FROM 1.9M POTASSIUM PHOSPHATE PH 6.8, THEN SOAKED FOR 3 HOURS IN 30 MM NEU5AC |
