2C42
Crystal Structure Of Pyruvate-Ferredoxin Oxidoreductase From Desulfovibrio africanus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-05-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.304, 145.981, 211.607 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.150 - 1.780 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kek |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.000 | 1.890 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Number of reflections | 233941 | |
| <I/σ(I)> | 16.54 | 5.02 |
| Completeness [%] | 91.7 | 75.9 |
| Redundancy | 4.42 | 3.46 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 10% PEG6000, 100MM MGCL2, 100MM NA CACODYLATE, 5% DTT, pH 6.00 |
