2C3F
The structure of a group A streptococcal phage-encoded tail-fibre showing hyaluronan lyase activity.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2003-11-15 |
| Detector | ADSC CCD |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 58.534, 58.534, 586.585 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 196.120 - 1.810 |
| R-factor | 0.19 |
| Rwork | 0.188 |
| R-free | 0.22300 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.581 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 195.520 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.100 | 0.260 |
| Number of reflections | 34533 | |
| <I/σ(I)> | 14 | 2.8 |
| Completeness [%] | 98.7 | 98.8 |
| Redundancy | 5.5 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 3.25M SODIUM FORMATE, PROTEIN CONCENTRATION 10-20 MG/ML IN 20MM HEPES 50MM CALCIUM CHLORIDE. 1 MICROLITRE OF MOTHER LIQUOR MIXED WITH 1 MICROLITRE OF PROTEIN. |
