2C2U
Dps from Deinococcus radiodurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-24 |
| Detector | ADSC CCD |
| Spacegroup name | P 2 3 |
| Unit cell lengths | 90.369, 90.369, 90.369 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.300 - 1.100 |
| R-factor | 0.127 |
| Rwork | 0.126 |
| R-free | 0.14900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c2f |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.702 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.310 | 1.130 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.060 | 0.480 |
| Number of reflections | 99021 | |
| <I/σ(I)> | 13.7 | 2.5 |
| Completeness [%] | 99.6 | 98.1 |
| Redundancy | 5.4 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 200MM LITHIUM SULFATE 100MM TRIS-HCL PH8.5 15% PEG4000, pH 8.50 |
