2C2G
Crystal structure of Threonine Synthase from Arabidopsis thaliana in complex with its cofactor pyridoxal phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-02-20 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 1 |
| Unit cell lengths | 57.626, 61.014, 76.678 |
| Unit cell angles | 108.96, 102.00, 107.36 |
Refinement procedure
| Resolution | 20.630 - 2.610 |
| R-factor | 0.191 |
| Rwork | 0.187 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e5x |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.708 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.750 |
| High resolution limit [Å] | 2.600 | 2.610 |
| Rmerge | 0.050 | 0.290 |
| Number of reflections | 25774 | |
| <I/σ(I)> | 6.8 | 2.5 |
| Completeness [%] | 95.5 | 93.5 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 1 M LICL, 13% (W/V) PEG 6000, 5 MM DTT, 0.1 M MES-KOH, pH 6.50 |
