2C1W
The structure of XendoU: a splicing independent snoRNA processing endoribonuclease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 164.451, 53.204, 133.466 |
| Unit cell angles | 90.00, 121.86, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.274 |
| Rwork | 0.274 |
| R-free | 0.27700 |
| Structure solution method | MIR |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.897 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE/RESOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.170 | 0.390 |
| Number of reflections | 64530 | |
| <I/σ(I)> | 8.8 | 4 |
| Completeness [%] | 96.0 | 92.8 |
| Redundancy | 4.3 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 1.6M AMMONIUM SULPHATE, 0.2M PHOSPHATE BUFFER PH5.5, pH 5.50 |
