2C1O
ENAIIHis Fab fragment in the free form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-08-12 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.805, 88.680, 141.441 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.750 |
R-factor | 0.2184 |
Rwork | 0.218 |
R-free | 0.30110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1f8t |
RMSD bond length | 0.007 |
RMSD bond angle | 1.456 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.800 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.060 | 0.300 |
Number of reflections | 23952 | |
<I/σ(I)> | 12.1 | 3.4 |
Completeness [%] | 95.6 | 83.3 |
Redundancy | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 20 % PEG3350, 0.1 M HEPES, pH 7.50 |