2C1M
Nup50:importin-alpha complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Collection date | 2004-03-10 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 74.467, 74.467, 188.023 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.200 |
R-factor | 0.2214 |
Rwork | 0.221 |
R-free | 0.25370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ial |
RMSD bond length | 0.006 |
RMSD bond angle | 1.150 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS (9.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.100 | 0.440 |
Number of reflections | 27445 | |
<I/σ(I)> | 5.5 | 1.9 |
Completeness [%] | 98.9 | 94.9 |
Redundancy | 7.6 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | DETAILS GIVEN IN PUBLICATION |