2C11
Crystal structure of the 2-hydrazinopyridine of semicarbazide- sensitive amine oxidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-05-14 |
Detector | ADSC CCD |
Spacegroup name | P 43 |
Unit cell lengths | 127.402, 127.402, 219.361 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.900 |
R-factor | 0.215 |
Rwork | 0.215 |
R-free | 0.27600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.500 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 83.300 | 3.100 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.160 | 0.460 |
Number of reflections | 72802 | |
<I/σ(I)> | 12.4 | 2 |
Completeness [%] | 94.6 | 90.6 |
Redundancy | 4.7 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 288 | THE PROTEIN WAS CRYSTALLISED IN 0.1 M KBR, 0.1 M ACETATE PH5, 38 % PEG1000 AT 288 K. THE CRYSTALS WERE SOAKED FOR FIVE DAYS IN 40 % PEG1000, 0.1 M ACETATE PH 5, 80 MM KBR, 5 MM CUCL2, 8 MM 2-HYDRAZINOPYRIDINE. |