2C10
The structure of a truncated, soluble version of semicarbazide- sensitive amine oxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-14 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 |
| Unit cell lengths | 130.236, 130.236, 221.529 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ksi |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.340 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.600 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.100 | 0.440 |
| Number of reflections | 126698 | |
| <I/σ(I)> | 13.8 | 3.2 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 5.2 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 288 | THE PROTEIN WAS CRYSTALLISED IN 0.1 M KBR, 0.1 M ACETATE PH 5, 38% PEG1000 AT 288 K. |
