2C0K
The structure of hemoglobin from the botfly Gasterophilus intestinalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-02-04 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 31 |
| Unit cell lengths | 47.697, 47.697, 145.245 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.850 - 2.600 |
| R-factor | 0.193 |
| Rwork | 0.186 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bk9 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.699 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 27.000 |
| High resolution limit [Å] | 2.600 |
| Rmerge | 0.090 |
| Number of reflections | 11160 |
| <I/σ(I)> | 7 |
| Completeness [%] | 98.0 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | PEG 4000 25%, 20 MM TRIS PH 7.5 |
