2C0E
Structure of PDI-related Chaperone, Wind with his-tag on C-terminus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-12-17 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.782, 50.368, 98.675 |
| Unit cell angles | 90.00, 112.19, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.350 |
| R-factor | 0.23 |
| Rwork | 0.226 |
| R-free | 0.29300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ovn |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.057 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.450 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.040 | 0.110 |
| Number of reflections | 19292 | |
| <I/σ(I)> | 20.05 | 9.46 |
| Completeness [%] | 98.3 | 99.2 |
| Redundancy | 4.11 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | PROTEIN: 15.0MG/ML WIND IN 5MM HEPES PH7.5, 25MM NACL, 0.0025%(V/V) BETA-MERCAPTOETHANOL RESERVOIR: 0.1M MES PH6.1, 50MM NACL, 18%(V/V) PEG 400 CRYO: 0.1M MES PH6.1, 25%(V/V) PEG 400, 10%(V/V)GLYCEROL, 50MM NACL, pH 6.20 |
