2C08
Rat endophilin A1 BAR domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-05 |
| Detector | ADSC CCD |
| Spacegroup name | I 41 |
| Unit cell lengths | 126.578, 126.578, 101.226 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 89.440 - 2.900 |
| R-factor | 0.28 |
| Rwork | 0.279 |
| R-free | 0.29500 |
| Structure solution method | MAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 0.956 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.160 | 0.950 |
| Number of reflections | 393336 | |
| <I/σ(I)> | 19.8 | 3.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 22.1 | 22.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 277 | 25% BUTANE-1,4-DIOL, 100MM TRIS PH 8.0,4DEGC, CRYOPROTECTED IN 40% BUTANE-1,4-DIOL, 25% ETHYLENE GLYCOL |
