2BZR

Crystal structure of accD5 (Rv3280), an acyl-CoA carboxylase beta- subunit from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 41 21 2
Unit cell lengths	173.562, 173.562, 339.823
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.090 - 2.200
R-factor	0.178
Rwork	0.176
R-free	0.21100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.768
Data scaling software	TRUNCATE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.220	2.220
High resolution limit [Å]	2.200	2.200
Rmerge	0.080	0.300
Number of reflections	251643
<I/σ(I)>	13.27	3.95
Completeness [%]	96.4	78.7
Redundancy	4.79	3.78

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		pH 7.00