2BZC
Oxidized and reduced structures of a mutant Plastocyanin of fern
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 |
| Unit cell lengths | 72.980, 72.980, 29.702 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.790 |
| R-factor | 0.193 |
| Rwork | 0.190 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kdi |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.539 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.900 | 1.890 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.060 | 0.230 |
| Number of reflections | 8720 | |
| <I/σ(I)> | 7.43 | 3.25 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 21.45 | 21.22 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 2.6 M AMMONIUM SULPHATE, 0.1M SODIUM ACETATE PH 4.5 |
