2BZ7
Oxidized and reduced structures of a mutant Plastocyanin of fern
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 |
| Unit cell lengths | 72.921, 72.921, 29.530 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 63.120 - 1.760 |
| R-factor | 0.193 |
| Rwork | 0.191 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kdj |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.505 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.570 | 1.780 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.050 | 0.160 |
| Number of reflections | 9095 | |
| <I/σ(I)> | 33.91 | 9.48 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 10.89 | 10.89 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 2.6 M AMMONIUM SULPHATE 0.1M SODIUM ACETATE PH 4.5 |
