2BYL
Structure of ornithine aminotransferase triple mutant Y85I Y55A G320F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-26 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 115.950, 115.950, 188.343 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.150 |
| R-factor | 0.161 |
| Rwork | 0.159 |
| R-free | 0.19100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oat |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.296 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.220 | 2.270 |
| High resolution limit [Å] | 2.000 | 2.150 |
| Rmerge | 0.090 | 0.300 |
| Number of reflections | 78515 | |
| <I/σ(I)> | 5.5 | 2.41 |
| Completeness [%] | 97.8 | 91.4 |
| Redundancy | 5.86 | 5.17 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.9 | 6-10%PEG6000, 120-160MM NACL, 50MM TRICINE PH7.9, 1MM DTT, pH 7.90 |
