2BYJ
Ornithine aminotransferase mutant Y85I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-09-01 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 115.723, 115.723, 186.551 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.880 - 3.020 |
| R-factor | 0.208 |
| Rwork | 0.207 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oat |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.989 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.700 | 3.160 |
| High resolution limit [Å] | 3.010 | 3.010 |
| Rmerge | 0.110 | 0.230 |
| Number of reflections | 28835 | |
| <I/σ(I)> | 5.96 | 3.16 |
| Completeness [%] | 98.8 | 92.9 |
| Redundancy | 2.9 | 2.76 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.9 | 6-10%PEG6000, 120-160MM NACL, 50MM TRICINE PH7.9, 1MM DTT, pH 7.90 |
