2BY1
Is radiation damage dependent on the dose-rate used during macromolecular crystallography data collection
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.608, 66.742, 153.538 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.550 |
| R-factor | 0.159 |
| Rwork | 0.158 |
| R-free | 0.19100 |
| Structure solution method | OTHER |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.739 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.630 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 89654 | |
| <I/σ(I)> | 11.4 | 1.7 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 20% PEG 2000 MME, 0.1 M TRIS-HCL PH 8.5, 5% GLYCEROL, 0.1 M MGCL2 |
