2BXJ

Double Mutant of the Ribosomal Protein S6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 64 2 2
Unit cell lengths	84.252, 84.252, 144.367
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.050 - 2.400
R-factor	0.198
Rwork	0.198
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lou
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.050	2.550
High resolution limit [Å]	2.400	2.400
Rmerge	0.120	0.430
Number of reflections	12515
<I/σ(I)>	23.56	6.9
Completeness [%]	97.0	95.2
Redundancy	18	12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.2M NA-CITRATE, 0.1M TRIS-CL PH 8.5, 25% (V/V) PEG400