2BWV
His361Ala Escherichia coli Aminopeptidase P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200H |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-04-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 177.339, 177.339, 96.475 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 154.300 - 1.700 |
| R-factor | 0.15 |
| Rwork | 0.150 |
| R-free | 0.16500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wl9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.206 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.230 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.060 | 0.480 |
| Number of reflections | 96986 | |
| <I/σ(I)> | 19.7 | 2.8 |
| Completeness [%] | 99.4 | 99.5 |
| Redundancy | 6 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | HANGING DROP VAPOUR DIFFUSION AT 4C. 2 UL 6 MG/ML APPRO PLUS 2 UL RESERVOIR SOLUTION: 28 % PEG 4K, 0.1 M TRIS PH 8.5. SOAKED IN RESERVOIR SOLUTION SUPPLEMENTED WITH 10% MPD AND 1 MM MNCL2 FOR 1 HR AT 4C PRIOR TO CRYOCOOLING. |
