2BWT
Asp260Ala Escherichia coli Aminopeptidase P
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-05-03 |
Detector | MARRESEARCH |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 137.588, 137.588, 231.948 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.890 - 2.900 |
R-factor | 0.171 |
Rwork | 0.169 |
R-free | 0.20200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bhc |
RMSD bond length | 0.008 |
RMSD bond angle | 1.074 |
Data reduction software | MOSFLM |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.130 | 3.060 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.100 | 0.530 |
Number of reflections | 22735 | |
<I/σ(I)> | 10.5 | 2 |
Completeness [%] | 91.7 | 94.5 |
Redundancy | 3.8 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | HANGING DROP VAPOUR DIFFUSION AT 4C. 2 UL 10 MG/ML APPRO PLUS 2 UL RESERVOIR SOLUTION: 30% MPD, 0.1 M CITRATE PH 7.5, 0.2 M MGACETATE. SOAKED IN RESERVOIR SOLUTION SUPPLEMENTED WITH AND 1 MM MNCL2 FOR 1 HR AT 4C PRIOR TO CRYOCOOLING. |