2BWS
His243Ala Escherichia coli Aminopeptidase P
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200H |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-06-08 |
Detector | MARRESEARCH |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 177.147, 177.147, 96.438 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 60.080 - 1.750 |
R-factor | 0.15 |
Rwork | 0.150 |
R-free | 0.16500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wl9 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.160 |
Data reduction software | MOSFLM |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 76.700 | 1.840 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.060 | 0.290 |
Number of reflections | 86631 | |
<I/σ(I)> | 17.4 | 3.4 |
Completeness [%] | 97.3 | 97.9 |
Redundancy | 5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 277 | HANGING DROP VAPOUR DIFFUSION AT 4C. 2 UL 6 MG/ML APPRO PLUS 2 UL RESERVOIR SOLUTION: 28 % PEG 4K, 0.1 M TRIS PH 8.2. SOAKED IN RESERVOIR SOLUTION SUPPLEMENTED WITH 10% MPD AND 1 MM MNCL2 FOR 1 HR AT 4C PRIOR TO CRYOCOOLING. |