2BWN

5-Aminolevulinate Synthase from Rhodobacter capsulatus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2003-11-06
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	67.875, 92.872, 250.131
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	129.100 - 2.100
R-factor	0.164
Rwork	0.161
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1BS0 AND 1FC4
RMSD bond length	0.021
RMSD bond angle	1.693
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.080	0.340
Number of reflections	370565
<I/σ(I)>	13.7	3.8
Completeness [%]	83.8	82.7
Redundancy	4.7	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		100 MM HEPES, PH 7.5, 200 MM NA ACETATE, 8% (V/V) ISOPROPANOL, 20% PEG 4000