2BWE
The crystal structure of the complex between the UBA and UBL domains of Dsk2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-22 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.361, 88.854, 141.497 |
| Unit cell angles | 90.00, 106.09, 90.00 |
Refinement procedure
| Resolution | 136.080 - 3.100 |
| R-factor | 0.241 |
| Rwork | 0.239 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | A B C D TETRAMER FROM PDB ENTRY 2BWB |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.538 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.200 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.100 | 0.500 |
| Number of reflections | 33693 | |
| <I/σ(I)> | 11.5 | 3.5 |
| Completeness [%] | 99.1 | 99.6 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 277 | 10-15% METHOXY PEG 5K BUFFERED WITH 0.1M MES PH 6.5 AT 4C |
