2BW8
Native structure of Endoglucanase 12A (Cel12A) from Rhodothermus marinus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2003-07-25 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.283, 67.867, 132.458 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.600 - 1.540 |
| R-factor | 0.157 |
| Rwork | 0.157 |
| R-free | 0.18000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1HOB |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.000 | 1.600 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.040 | 0.290 |
| Number of reflections | 75552 | |
| <I/σ(I)> | 23.6 | 5 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 6.9 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1M MES PH 6.5, 1.5M (NH4)2SO4, 0.2M LI2SO4, 10MG/ML PROTEIN |
