2BW5
Atomic Resolution Structure of NO-bound Achromobacter cycloclastes Cu Nitrite Reductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-30 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 95.334, 95.334, 95.334 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.120 |
| R-factor | 0.129 |
| Rwork | 0.128 |
| R-free | 0.16400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bw4 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.136 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.500 | 1.160 |
| High resolution limit [Å] | 1.120 | 1.120 |
| Rmerge | 0.050 | 0.400 |
| Number of reflections | 108076 | |
| <I/σ(I)> | 19.5 | 2.5 |
| Completeness [%] | 97.8 | 88 |
| Redundancy | 4.4 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.75 | 1.6 M SODIUM PHOSPHATE, 100MM SODIUM ACETATE, PH 4.75 |
