2BVZ
Mutant of the Ribosomal Protein S6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 52.605, 106.004, 41.138 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.560 - 2.200 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lou |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.560 | 2.340 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.080 | 0.320 |
| Number of reflections | 6127 | |
| <I/σ(I)> | 5 | 5 |
| Completeness [%] | 96.3 | 92.2 |
| Redundancy | 7.8 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2M NA-CITRATE, 0.1M TRIS-CL PH 8.5, 17.5% (V/V) PEG400 |
