2BVN

E. coli EF-Tu:GDPNP in complex with the antibiotic enacyloxin IIa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Detector technology	CCD
Collection date	2003-05-25
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	72.070, 72.070, 335.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.060 - 2.300
R-factor	0.23
Rwork	0.230
R-free	0.27100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ob2
RMSD bond length	0.007
RMSD bond angle	1.410
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.350
High resolution limit [Å]	2.300	2.300
Rmerge	0.100	0.540
Number of reflections	37737
<I/σ(I)>	24.1	2.1
Completeness [%]	92.5	53.8
Redundancy	10	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	292	2 MICROL PROTEIN SOLUTION MIXED WITH 1 MICROL RESERVOIR SOLUTION (450 MM NACL. 22% PEG6000, 6% GLYCEROL, 7 MM MGCL2, 100 MM TRIS-HCL PH 7.5), SITTING DROP 19 DEG. C