2BVA

Crystal structure of the human P21-activated kinase 4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2005-04-23
Detector	MARRESEARCH
Spacegroup name	P 32
Unit cell lengths	118.110, 118.110, 55.530
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.1878
R-free	0.26860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u5r
RMSD bond length	0.007
RMSD bond angle	0.023
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.400	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.060	0.280
Number of reflections	38417
<I/σ(I)>	11.86	3.55
Completeness [%]	99.8	100
Redundancy	2.7	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP			SITTING DROPS, 1.5 M NACL, 10% ETHANOL