2BTU
Crystal structure of Phosphoribosylformylglycinamidine cyclo-ligase from Bacillus Anthracis at 2.3A resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-25 |
| Detector | ADSC CCD |
| Spacegroup name | P 41 |
| Unit cell lengths | 89.490, 89.490, 88.117 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.310 |
| R-free | 0.24910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cli |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.020 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.390 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Rmerge | 0.060 | 0.460 |
| Number of reflections | 28479 | |
| <I/σ(I)> | 20.4 | 1.4 |
| Completeness [%] | 93.3 | 47.9 |
| Redundancy | 4.1 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | HANGING DROP VAPOUR DIFFUSION, 17% PEG2K, 0.1M TRIS-HCL PH 8.0, 0.1M TMAO (TRIMETHYLAMINE-N-OXIDE) |
