2BTQ
Structure of BtubAB heterodimer from Prosthecobacter dejongeii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-08 |
| Detector | ADSC CCD |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 154.398, 154.398, 256.330 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 53.520 - 3.200 |
| R-factor | 0.2147 |
| Rwork | 0.215 |
| R-free | 0.24660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bto |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.376 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.520 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.090 | 0.380 |
| Number of reflections | 29998 | |
| <I/σ(I)> | 12.8 | 2.6 |
| Completeness [%] | 99.0 | 99.5 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 1.5M LI2SO4, 0.4M TRIS/HCL, PH 7.5 |
