2BTL
Crystal structure of the N-terminal domain of IBV coronavirus nucleocapsid
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-20 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.770, 35.370, 55.950 |
| Unit cell angles | 100.51, 95.48, 110.16 |
Refinement procedure
| Resolution | 20.000 - 1.950 |
| R-factor | 0.23 |
| Rwork | 0.227 |
| R-free | 0.27600 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.144 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.050 | 0.120 |
| Number of reflections | 76265 | |
| <I/σ(I)> | 8.6 | 3.7 |
| Completeness [%] | 96.6 | 95 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
