2BT8
Structure of the C-terminal receptor-binding domain of avian reovirus fibre sigmaC, space group P6322.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-27 |
| Detector | MARRESEARCH |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 75.598, 75.598, 243.139 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 121.270 - 3.000 |
| R-factor | 0.203 |
| Rwork | 0.198 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.731 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.160 |
| High resolution limit [Å] | 2.710 | 3.000 |
| Rmerge | 0.080 | 0.260 |
| Number of reflections | 10910 | |
| <I/σ(I)> | 8.2 | 2.9 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 8.8 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 100 MM TRIS-HCL PH 8.5, 1.5 M AMMONIUM SULPHATE, 12% GLYCEROL |
