2BT7
Structure of the C-terminal receptor-binding domain of avian reovirus fibre sigmaC, Cd crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-30 |
| Detector | MARRESEARCH |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 73.075, 73.075, 69.928 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 69.840 - 2.350 |
| R-factor | 0.189 |
| Rwork | 0.183 |
| R-free | 0.24300 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.608 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.500 | 2.450 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.080 | 0.340 |
| Number of reflections | 9602 | |
| <I/σ(I)> | 7.1 | 2.1 |
| Completeness [%] | 95.8 | 71.6 |
| Redundancy | 10.5 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 100 MM TRIS-HCL PH 8.5, 1.5 M AMMONIUM SULPHATE, 12 % GLYCEROL, 10 MM CADMIUM SULPHATE |
