2BSQ
FitAB bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-08 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.040, 82.403, 135.503 |
| Unit cell angles | 90.00, 94.19, 90.00 |
Refinement procedure
| Resolution | 70.360 - 3.000 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1YH4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.650 | 3.150 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.180 | 0.440 |
| Number of reflections | 16708 | |
| <I/σ(I)> | 3.1 | 1.5 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.4 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 0.1 M ACETATE, PH 4.0 7.2 % PEG 20,000 7.2 % PEG 550 MME 0.26 M NA ACETATE, PH 7.0 |
