2BSL
Crystal structure of L. lactis dihydroorotate dehydrogense A in complex with 3,4-dihydroxybenzoate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.588, 108.160, 66.222 |
| Unit cell angles | 90.00, 104.06, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.300 |
| R-factor | 0.189 |
| Rwork | 0.186 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ovd |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.687 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.320 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.060 | 0.230 |
| Number of reflections | 32557 | |
| <I/σ(I)> | 22.9 | 5.6 |
| Completeness [%] | 90.6 | 62.8 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 17 MG/ML PROTEIN AND 2.6 MM 3, 4-DIOHB HANGING DROPS 2 MICROLITERS OF PROTEIN AND 2 MICROLITERS RESERVOIR: 30 % PEG 6000, 1 MM DTT, 0.2 M SODIUM ACETATE AND 0.1 M TRIS-HCL PH 8.5 |
