2BSJ
Native Crystal Structure of the Type III Secretion chaperone SycT from Yersinia enterocolitica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-18 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.112, 79.562, 52.093 |
| Unit cell angles | 90.00, 101.21, 90.00 |
Refinement procedure
| Resolution | 79.560 - 1.830 |
| R-factor | 0.186 |
| Rwork | 0.184 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2BSH 2bsi |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.484 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.500 | 1.860 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.060 | 0.380 |
| Number of reflections | 23529 | |
| <I/σ(I)> | 14.8 | 2.1 |
| Completeness [%] | 97.6 | 83.5 |
| Redundancy | 5.6 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M SODIUM CITRATE PH 5.6, 5 % ISOPROPANOL, 21 % POLYETHYLENE GLYCOL 4000, HANGING-DROP VAPOR DIFFUSION AT 4 DEGREE CELSIUS |
