2BSH
Crystal structure of the type III secretion chaperone SycT from Yersinia enterocolitica (crystal form 2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 62 |
| Unit cell lengths | 92.040, 92.040, 55.370 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 79.810 - 1.900 |
| R-factor | 0.176 |
| Rwork | 0.174 |
| R-free | 0.22000 |
| Structure solution method | SAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.875 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.810 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.400 | |
| Number of reflections | 21134 | |
| <I/σ(I)> | 16.6 | 5.53 |
| Completeness [%] | 99.5 | 96.4 |
| Redundancy | 11.2 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 1.8 M AMMONIUM SULFATE, 0.1 M SODIUM BICARBONATE PH 10.5, 50 MM MAGNESIUM CHLORIDE HANGING-DROP VAPOR DIFFUSION AT 4 CELSIUS DEGREE |
