2BSF
Structure of the C-terminal receptor-binding domain of avian reovirus fibre sigmaC, Zn crystal form.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.28191,1.15872, 1.28271, 1.28194 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 74.777, 74.777, 74.639 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 74.540 - 2.100 |
| R-factor | 0.167 |
| Rwork | 0.161 |
| R-free | 0.22300 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.750 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.070 | 0.290 |
| Number of reflections | 14231 | |
| <I/σ(I)> | 7.3 | 2.6 |
| Completeness [%] | 98.7 | 97.7 |
| Redundancy | 7.8 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 100 MM TRIS-HCL PH 8.5, 1.5 M AMMONIUM SULPHATE, 12% GLYCEROL, 10 MM ZINC SULPHATE |
