2BS8
Crystal structure of F17b-G in complex with N-acetyl-D-glucosamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-20 |
| Detector | MARRESEARCH |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 96.506, 96.506, 97.877 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.910 - 2.250 |
| R-factor | 0.3317 |
| Rwork | 0.332 |
| R-free | 0.39720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o9w |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.000 | 2.320 |
| High resolution limit [Å] | 2.240 | 2.240 |
| Rmerge | 0.120 | 0.210 |
| Number of reflections | 11000 | |
| <I/σ(I)> | 10 | 5 |
| Completeness [%] | 96.8 | 91.1 |
| Redundancy | 5.1 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | HANGING DROP: 1 MICROLITER OF 10% ETHANOL WITH 1.5 M NACL PLUS 1 MICROLITER OF 16 MG/ML F17BG |
